Screening of Transient Receptor Potential Vannilloid-1 (TRPV1), TRPV3 & TRPA1 antagonists for indication of

pain

● Executing screening (Invivo) NCE of:

o CFA induced thermal (Hargreave’s method) hyperalgesia

o CFA induced mechanical (Randall selitto method) hyperalgesia

o Capsaicin induced hypothermia

● Investigated NCE’s in model of osteoarthritis (Monosodium iodoacetate induced osteoarthritis in rats)

● Tested antagonists in pSNL, CCI, SNL and Formalin paw licking pain models

● Performing exvivo screening of these NCE using capsaicin induced rat urinary bladder contraction

Investigation of PDE4, RORγt and NLRP3 inhibitors for potential effects in rheumatoid arthritis, inflammatory and

neuropathic pain

● Developing animal models of arthritis:

o Collagen Induced Arthritis (CIA) in male DBA1/J mice and female Lewis rats

o Adjuvant induced arthritis model in female Lewis rats

o Carrageenan induced air pouch model of inflammation

Investigation of NLRP3 Inhibitors in EAE model of Multiple Sclerosis

● Developing EAE model of Multiple Sclerosis:

o Standardisation and Validation of Experimental Autoimmune Encephalomyelitis in C57BL/6J mice

o Cytokine estimation in spinal cord of EAE mice by ELISA

o Flow cytometric analysis of T cell infiltration in spinal cord of EAE mice

Screening of cannabinoid 2 (CB2) agonists for the indication of pain

● Screening NCE’s (Invitro) by receptor binding assay in rat and mouse spleen membrane for its affinity towards CB2

receptor

● Investigating activity of NCE using various inflammatory and neuropathic pain models like CFA and CCI induced

mechanical hyperalgesia in rats

● Evaluating activities in CIA and carrageenan air pouch models

Investigation of RORγt inhibitors for potential effects in chronic obstructive pulmonary disease

● Developing animal models of COPD:

o Viral COPD exacerbation: CS + Poly IC induced COPD exacerbation in female C57BL/6J mice

o Acute cigarette smoke induced pulmonary inflammation in female C57BL/6J mice

o LPS induced pulmonary inflammation in female Balb/c mice and female SD rats

o Anti-CD3 antibody induced IL-17 in male C57BL/6J mice

Screening of PDE10A inhibitors for Schizophrenia

● Spearheading:

o Apomorphine induced hypothermia in mice

o Apomorphine induced climbing behaviour in mice

o Apomorphine induced stereotypic behaviour in rats

o Quipazine induced head twitches in rats

o MK 801 induced psychosis behaviour in rats

o Assessment of cataleptic behaviour in rats

o Conditioned Avoidance Response in rats

o cGMP and cAMP estimation in striatum and hippocampus regions of rat brain

Biomarker Estimation and ELISA Assays

● Managing:

o IL-1β estimation in paw tissue of arthritic mice and rats

o IL-17 estimation in paw tissue of arthritic mice and rats

Safety Pharmacological Studies

Testing the NCE’s in various safety pharmacological model

● Rota rod test in rats

● Change in rectal temperature of rats and mice

● Tail flick test for rats and mice

● Hot plate test for mice